Fast spatiotemporal smoothing of calcium measurements in dendritic trees.

ABSTRACT: We discuss methods for fast spatiotemporal smoothing of calcium signals in dendritic trees, given single-trial, spatially localized imaging data obtained via multi-photon microscopy. By analyzing the dynamics of calcium binding to probe molecules and the effects of the imaging procedure, we show that calcium concentration can be estimated up to an affine transformation, i.e., an additive and multiplicative constant. To obtain a full spatiotemporal estimate, we model calcium dynamics within the cell using a functional approach. The evolution of calcium concentration is represented through a smaller set of hidden variables that incorporate fast transients due to backpropagating action potentials (bAPs), or other forms of stimulation. Because of the resulting state space structure, inference

SUBMITTER: Pnevmatikakis EA 

PROVIDER: S-EPMC3386185 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature