Use of mutagenesis, genetic mapping and next generation transcriptomics to investigate insecticide resistance mechanisms.

ABSTRACT: Insecticide resistance is a worldwide problem with major impact on agriculture and human health. Understanding the underlying molecular mechanisms is crucial for the management of the phenomenon; however, this information often comes late with respect to the implementation of efficient counter-measures, particularly in the case of metabolism-based resistance mechanisms. We employed a genome-wide insertional mutagenesis screen to Drosophila melanogaster, using a Minos-based construct, and retrieved a line (MiT[w(-)]3R2) resistant to the neonicotinoid insecticide Imidacloprid. Biochemical and bioassay data indicated that resistance was due to increased P450 detoxification. Deep sequencing transcriptomic analysis revealed substantial over- and under-representation of 357 transcripts in the re

SUBMITTER: Kalajdzic P 

PROVIDER: S-EPMC3386967 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature