Oligonucleotide primers for targeted amplification of single-copy nuclear genes in apocritan Hymenoptera.

ABSTRACT:

Background

Published nucleotide sequence data from the mega-diverse insect order Hymenoptera (sawflies, bees, wasps, and ants) are taxonomically scattered and still inadequate for reconstructing a well-supported phylogenetic tree for the order. The analysis of comprehensive multiple gene data sets obtained via targeted PCR could provide a cost-effective solution to this problem. However, oligonucleotide primers for PCR amplification of nuclear genes across a wide range of hymenopteran species are still scarce.

Findings

Here we present a suite of degenerate oligonucleotide primer pairs for PCR amplification of 154 single-copy nuclear protein-coding genes from Hymenoptera. These primers were inferred from genome sequence data from nine Hymenoptera (seven species of ants, the h

SUBMITTER: Hartig G 

PROVIDER: S-EPMC3387199 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature