Localization and functional characterization of the rat Oatp4c1 transporter in an in vitro cell system and rat tissues.

ABSTRACT: The organic anion transporting polypeptide 4c1 (Oatp4c1) was previously identified as a novel uptake transporter predominantly expressed at the basolateral membrane in the rat kidney proximal tubules. Its functional role was suggested to be a vectorial transport partner of an apically-expressed efflux transporter for the efficient translocation of physiological substrates into urine, some of which were suggested to be uremic toxins. However, our in vitro studies with MDCKII cells showed that upon transfection rat Oatp4c1 polarizes to the apical membrane. In this report, we validated the trafficking and function of Oatp4c1 in polarized cell systems as well as its subcellular localization in rat kidney. Using several complementary biochemical, molecular and proteomic methods as well as antib

SUBMITTER: Kuo KL 

PROVIDER: S-EPMC3387246 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature