Transient supplementation of anabolic growth factors rapidly stimulates matrix synthesis in engineered cartilage.

ABSTRACT: The purpose of the presented work is to examine the response of engineered cartilage to a transient, 2-week application of anabolic growth factors compared to continuous exposure in in vitro culture. Immature bovine chondrocytes were suspended in agarose hydrogel and cultured for 28 days (Study 1) or 42 days (Study 2) in chondrogenic media with TGF-β1, TGF-β3, or IGF-I either added for only the first 14 days in culture or added to the media for the entire study period. In both studies, there were no statistical differences in tissue mechanical or biochemical properties between the growth factors on day 14. In Study 1, growth factor removal led to a significant and drastic increase in Young's modulus and glycosaminoglycans content compared to continuously exposed controls on day 28. In Stud

SUBMITTER: Ng KW 

PROVIDER: S-EPMC3387273 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature