Strategies for assessing proton linkage to bimolecular interactions by global analysis of isothermal titration calorimetry data.

ABSTRACT: Isothermal titration calorimetry (ITC) is a traditional and powerful method for studying the linkage of ligand binding to proton uptake or release. The theoretical framework has been developed for more than two decades and numerous applications have appeared. In the current work, we explored strategic aspects of experimental design. To this end, we simulated families of ITC data sets that embed different strategies with regard to the number of experiments, range of experimental pH, buffer ionization enthalpy, and temperature. We then re-analyzed the families of data sets in the context of global analysis, employing a proton linkage binding model implemented in the global data analysis platform SEDPHAT, and examined the information content of all data sets by a detailed statistical error an

SUBMITTER: Coussens NP 

PROVIDER: S-EPMC3388511 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature