γ-Secretase inhibitor enhances antitumour effect of radiation in Notch-expressing lung cancer.

ABSTRACT:

Background

Notch receptor has an important role in both development and cancer. We previously reported that inhibition of the Notch3 by γ-secretase inhibitor (GSI) induces apoptosis and suppresses tumour proliferation in non-small-cell lung cancer. Although radiation is reported to induce Notch activation, little is known about the relationship between radiation and Notch pathway.

Methods

We examined the effect of combining GSI and radiation at different dosing in three Notch expressing lung cancer cell lines. The cytotoxic effect of GSI and radiation was evaluated using MTT assay and clonogenic assay in vitro and xenograft models. Expressions of Notch pathway, mitogen-activated protein kinase (MAPK) pathway and Bcl-2 family proteins were investigated using western blot anal

SUBMITTER: Mizugaki H 

PROVIDER: S-EPMC3388558 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature