Identification of lipids and lipid-binding proteins in phloem exudates from Arabidopsis thaliana.

ABSTRACT: The phloem plays a crucial role in assimilate and nutrient transport, pathogen response, and plant growth and development. Yet, few species have yielded pure phloem exudate and, if proteins need to be analysed, those species may not have sequenced genomes, making identification difficult. The enrichment of Arabidopsis thaliana phloem exudate in amounts large enough to allow for metabolite and protein analysis is described. Using this method, it was possible to identify 65 proteins present in the Arabidopsis phloem exudate. The majority of these proteins could be grouped by response to pathogens, stress, or hormones, carbon metabolism, protein interaction, modification, and turnover, and transcription factors. It was also possible to detect 11 proteins that play a role in lipid/fatty acid m

SUBMITTER: Guelette BS 

PROVIDER: S-EPMC3388829 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature