Expression and regulation of corticotropin-releasing factor receptor type 2β in developing and mature mouse skeletal muscle.

ABSTRACT: Corticotropin-releasing factor receptor type 2 (CRFR2) is highly expressed in skeletal muscle (SM) tissue where it is suggested to inhibit interactions between insulin signaling pathway components affecting whole-body glucose homeostasis. However, little is known about factors regulating SM CRFR2 expression. Here, we demonstrate the exclusive expression of CRFR2, and not CRFR1, in mature SM tissue using RT-PCR and ribonuclease protection assays and report a differential expression of CRF receptors during C2C12 myogenic differentiation. Whereas C2C12 myoblasts exclusively express CRFR1, the C2C12 myotubes solely express CRFR2. Using cAMP luciferase assays and calcium mobilization measurements, we further demonstrate the functionality of these differentially expressed receptors. Using lucife

SUBMITTER: Kuperman Y 

PROVIDER: S-EPMC3388898 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature