Expression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii.

ABSTRACT: Acinetobacter baumannii is a common multidrug-resistant clinical pathogen that is often found in hospitals. The A. baumannii phosphoglycerate kinase (AbPGK) is involved in the key energy-producing pathway of glycolysis and presents a potential target for antibiotic development. AbPGK has been expressed and purified; it was crystallized using lithium sulfate as the precipitant. The AbPGK crystals belonged to space group P222(1). They diffracted to a resolution of 2.5?Å using synchrotron radiation at the Canadian Light Source.

SUBMITTER: Baretta K 

PROVIDER: S-EPMC3388923 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature