Improved serodiagnosis of cystic echinococcosis using the new recombinant 2B2t antigen.

ABSTRACT: A standardized test for the serodiagnosis of human cystic echinococcosis (CE) is still needed, because of the low specificity and sensitivity of the currently available commercial tools and the lack of proper evaluation of the existing recombinant antigens. In a previous work, we defined the new ELISA-B2t diagnostic tool for the detection of specific IgGs in CE patients, which showed high sensitivity and specificity, and was useful in monitoring the clinical evolution of surgically treated CE patients. Nevertheless, this recombinant antigen gave rise to false-negative results in a percentage of CE patients. Therefore, in an attempt to improve its sensitivity, we constructed B2t-derived recombinant antigens with two, four and eight tandem repeat of B2t units, and tested them by ELISA on ser

SUBMITTER: Hernandez-Gonzalez A 

PROVIDER: S-EPMC3389031 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature