Automated imaging reveals a concentration dependent delay in reversibility of cardiac myocyte hypertrophy.

ABSTRACT: Cardiac hypertrophy is controlled by a dense signaling network with many pathways associated with cardiac myocyte growth. New large scale methodology is required to quantitatively characterize the pathways that distinguish reversible forms of hypertrophy from irreversible forms that lead to heart failure. Our automated image acquisition method records 5×5 mosaic images of fluorescent protein-labeled cardiac myocytes within each well of a 96-well plate using an automated stage and focus. Post-processing algorithms automatically identify cell edges, quantify cell phenotypes, and track cells. We uniquely applied our imaging platform to study hypertrophy reversibility in a scalable cell model. Cell area changes after washout of a dose response to the α-adrenergic receptor (αAR) agonist phenyle

SUBMITTER: Ryall KA 

PROVIDER: S-EPMC3389167 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature