CXCR4 activation maintains a stem cell population in tamoxifen-resistant breast cancer cells through AhR signalling.

ABSTRACT:

Background

Tamoxifen is commonly used for breast cancer therapy. However, tamoxifen resistance is an important clinical problem. Continuous treatment with conventional therapy may contribute to cancer progression in recurring cancers through the accumulation of drug-resistant cancer progenitors.

Methods

To investigate signalling mechanisms important for the maintenance and viability of drug-resistant cancer progenitors, we used microarray analysis, PCR array for genes involved in cancer drug resistance and metabolism, flow cytometry, soft agar colony formation assay, in vivo tumourigenicity assay and immunohistochemical analysis using tamoxifen-sensitive and tamoxifen-resistant breast cancer MCF7 cells.

Results

Downregulation of CXCR4 signalling by small molecule anta

SUBMITTER: Dubrovska A 

PROVIDER: S-EPMC3389396 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature