2D difference gel electrophoresis analysis of different time points during the course of neoplastic transformation of human mammary epithelial cells.

ABSTRACT: Cell culture models of oncogenesis that use cellular reprogramming to generate a neoplastic cell from a normal cell provide one of the few opportunities to study the early stages of breast cancer development. Human mammary epithelial cells (HMECs) were induced to undergo a neoplastic transformation using defined genetic elements to generate transformed HMECs (THMECs). To identify proteins that displayed significantly different levels of abundance at three consecutive time points in oncogenesis over an 80 day period, protein extracts were analyzed by two-dimensional difference gel electrophoresis (2D-DIGE). Nine proteins were found to be significantly different in abundance: keratin 1, keratin 7, heat shock protein 4A-like, t-complex protein 1, stathmin, gelsolin, FK506 binding protein 5, r

SUBMITTER: DeAngelis JT 

PROVIDER: S-EPMC3390236 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature