The molecular basis of ubiquitin-like protein NEDD8 deamidation by the bacterial effector protein Cif.

ABSTRACT: The cycle inhibiting factors (Cifs) are a family of translocated effector proteins, found in diverse pathogenic bacteria, that interfere with the host cell cycle by catalyzing the deamidation of a specific glutamine residue (Gln40) in NEDD8 and the related protein ubiquitin. This modification prevents recycling of neddylated cullin-RING ligases, leading to stabilization of various cullin-RING ligase targets, and also prevents polyubiquitin chain formation. Here, we report the crystal structures of two Cif/NEDD8 complexes, revealing a conserved molecular interface that defines enzyme/substrate recognition. Mutation of residues forming the interface suggests that shape complementarity, rather than specific individual interactions, is a critical feature for complex formation. We show that Cif

SUBMITTER: Crow A 

PROVIDER: S-EPMC3390873 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature