Cross-comparison of protein recognition of sialic acid diversity on two novel sialoglycan microarrays.

ABSTRACT: DNA and protein arrays are commonly accepted as powerful exploratory tools in research. This has mainly been achieved by the establishment of proper guidelines for quality control, allowing cross-comparison between different array platforms. As a natural extension, glycan microarrays were subsequently developed, and recent advances using such arrays have greatly enhanced our understanding of protein-glycan recognition in nature. However, although it is assumed that biologically significant protein-glycan binding is robustly detected by glycan microarrays, there are wide variations in the methods used to produce, present, couple, and detect glycans, and systematic cross-comparisons are lacking. We address these issues by comparing two arrays that together represent the marked diversity of s

SUBMITTER: Padler-Karavani V 

PROVIDER: S-EPMC3391140 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature