Low level genome mistranslations deregulate the transcriptome and translatome and generate proteotoxic stress in yeast.

ABSTRACT:

Background

Organisms use highly accurate molecular processes to transcribe their genes and a variety of mRNA quality control and ribosome proofreading mechanisms to maintain intact the fidelity of genetic information flow. Despite this, low level gene translational errors induced by mutations and environmental factors cause neurodegeneration and premature death in mice and mitochondrial disorders in humans. Paradoxically, such errors can generate advantageous phenotypic diversity in fungi and bacteria through poorly understood molecular processes.

Results

In order to clarify the biological relevance of gene translational errors we have engineered codon misreading in yeast and used profiling of total and polysome-associated mRNAs, molecular and biochemical tools to characteri

SUBMITTER: Paredes JA 

PROVIDER: S-EPMC3391182 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature