Quantifying insulin receptor isoform expression in FFPE breast tumors.

ABSTRACT:

Background

The development of predictive biomarkers for IGF targeted anti-cancer therapeutics remains a critical unmet need. The insulin receptor A isoform (InsR-A) has been identified as a possible biomarker candidate but quantification of InsR-A in widely available formalin fixed paraffin embedded (FFPE) tissues is complicated by its similarities with the metabolic signaling insulin receptor isoform B (InsR-B). In the present study, qPCR based assays specific for InsR-A, InsR-B and IGF-1R were developed for use in FFPE tissues and tested for feasible use in clinical archived FFPE estrogen receptor (ER)+and ER- breast cancer tumors.

Design

FFPE compatible primer sets were designed with amplicon sizes of less than 60 base pairs and validated for target specificity, assay rep

SUBMITTER: Harrington SC 

PROVIDER: S-EPMC3392524 | biostudies-literature | 2012 Jun-Aug

REPOSITORIES: biostudies-literature