Genome-wide transcriptome analysis in murine neural retina using high-throughput RNA sequencing.

ABSTRACT: Genome-wide characterization of the retinal transcriptome is central to understanding development, physiology and disorders of the visual system. Massively parallel, short-read sequencing of mRNA libraries was used to generate an extensive map of the transcriptome of the adult, murine neural retina. RNA-seq data strongly corroborates prior transcriptome studies by microarray and SAGE. However, several novel features of the retinal transcriptome were discovered. For example, retinal disease genes were discovered to be among the most highly expressed in the transcriptome. We also demonstrate other interesting features of the retinal transcriptome, for example, that the retina appears to employ a very specific and restricted set of synaptic vesicle genes, and also that there is persistence of

SUBMITTER: Gamsiz ED 

PROVIDER: S-EPMC3392719 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature