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Nucleobase recognition at alkaline pH and apparent pKa of single DNA bases immobilised within a biological nanopore.

ABSTRACT: The four DNA bases are recognized in immobilized DNA strands at high alkaline pH by nanopore current recordings. Ionic currents through the biological nanopores are also employed to measure the apparent pK(a) values of single nucleobases within the immobilised DNA strands.

SUBMITTER: Franceschini L

PROVIDER: S-EPMC3393037 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Nucleobase recognition at alkaline pH and apparent pKa of single DNA bases immobilised within a biological nanopore.

Franceschini Lorenzo L Mikhailova Ellina E Bayley Hagan H Maglia Giovanni G

Chemical communications (Cambridge, England) 20111117 10

The four DNA bases are recognized in immobilized DNA strands at high alkaline pH by nanopore current recordings. Ionic currents through the biological nanopores are also employed to measure the apparent pK(a) values of single nucleobases within the immobilised DNA strands. ...[more]

PMID: 22089628

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