MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease.

ABSTRACT: The protein encoded by MAP1272c has been shown to be an antigen of Mycobacterium avium subsp. paratuberculosis that contains an NlpC/P60 superfamily domain found in lipoproteins or integral membrane proteins. Proteins containing this domain have diverse enzymatic functions that include peptidases, amidases, and acetyltransferases. The NlpC protein was examined in comparison to over 100 recombinant proteins and showed the strongest antigenicity when analyzed with sera from cattle with Johne's disease. To further localize the immunogenicity of NlpC, recombinant proteins representing defined regions were expressed and evaluated with sera from cattle with Johne's disease. The region from amino acids 74 to 279 was shown to be the most immunogenic. This fragment was also evaluated against a comm

SUBMITTER: Bannantine JP 

PROVIDER: S-EPMC3393375 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature