Ecdysone induced gene expression is associated with acetylation of histone H3 lysine 23 in Drosophila melanogaster.

ABSTRACT: Posttranslational modification of histones regulates transcription but the exact role that acetylation of specific lysine residues plays in biological processes in vivo is still not clearly understood. To assess the contribution of different histone modifications to transcriptional activation in vivo, we determined the acetylation patterns on the ecdysone induced Eip74EF and Eip75B genes in Drosophila melanogaster larvae by chromatin immunoprecipitation. We found that acetylation of histone H3 lysine 23 is localized to promoters and correlates with endogenous ecdysone induced gene activation. In contrast, acetylation of lysines 8, 12 and 16 of histone H4 and lysine 9 of histone H3 showed minor differences in their distribution on the regulatory and transcribed regions tested, and had limit

SUBMITTER: Bodai L 

PROVIDER: S-EPMC3393682 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature