A novel flow cytometric HTS assay reveals functional modulators of ATP binding cassette transporter ABCB6.

ABSTRACT: ABCB6 is a member of the adenosine triphosphate (ATP)-binding cassette family of transporter proteins that is increasingly recognized as a relevant physiological and therapeutic target. Evaluation of modulators of ABCB6 activity would pave the way toward a more complete understanding of the significance of this transport process in tumor cell growth, proliferation and therapy-related drug resistance. In addition, this effort would improve our understanding of the function of ABCB6 in normal physiology with respect to heme biosynthesis, and cellular adaptation to metabolic demand and stress responses. To search for modulators of ABCB6, we developed a novel cell-based approach that, in combination with flow cytometric high-throughput screening (HTS), can be used to identify functional modula

SUBMITTER: Polireddy K 

PROVIDER: S-EPMC3393737 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature