Profiling cellular protein complexes by proximity ligation with dual tag microarray readout.

ABSTRACT: Patterns of protein interactions provide important insights in basic biology, and their analysis plays an increasing role in drug development and diagnostics of disease. We have established a scalable technique to compare two biological samples for the levels of all pairwise interactions among a set of targeted protein molecules. The technique is a combination of the proximity ligation assay with readout via dual tag microarrays. In the proximity ligation assay protein identities are encoded as DNA sequences by attaching DNA oligonucleotides to antibodies directed against the proteins of interest. Upon binding by pairs of antibodies to proteins present in the same molecular complexes, ligation reactions give rise to reporter DNA molecules that contain the combined sequence information from

SUBMITTER: Hammond M 

PROVIDER: S-EPMC3393744 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature