Splice variant PRKC-ζ(-PrC) is a novel biomarker of human prostate cancer.

ABSTRACT:

Background

Previously, using gene-knockdown techniques together with genome expression array analysis, we showed the gene protein Kinase C (PKC)-zeta (PRKCZ) to mediate the malignant phenotype of human prostate cancer. However, according to NCBI, the gene has undergone several major iterations. Therefore, to understand the relationship between its structure and biological activities, we have analysed its expressed sequence in prostate cancer cell lines and tissues.

Methods

Transcriptome-walking and targeted PCR were used to sequence the mRNA transcribed from PRKCZ. Hydropathy analysis was employed to analyse the hypothetical protein sequence subsequently translated and to identify an appropriate epitope to generate a specific monoclonal antibody.

Results

A novel seque

SUBMITTER: Yao S 

PROVIDER: S-EPMC3394965 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature