High-throughput identification and screening of novel Methylobacterium species using whole-cell MALDI-TOF/MS analysis.

ABSTRACT: Methylobacterium species are ubiquitous α-proteobacteria that reside in the phyllosphere and are fed by methanol that is emitted from plants. In this study, we applied whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis (WC-MS) to evaluate the diversity of Methylobacterium species collected from a variety of plants. The WC-MS spectrum was reproducible through two weeks of cultivation on different media. WC-MS spectrum peaks of M. extorquens strain AM1 cells were attributed to ribosomal proteins, but those were not were also found. We developed a simple method for rapid identification based on spectra similarity. Using all available type strains of Methylobacterium species, the method provided a certain threshold similarity value for species-leve

SUBMITTER: Tani A 

PROVIDER: S-EPMC3395638 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature