Isolation and genomic analysis of circulating tumor cells from castration resistant metastatic prostate cancer.

ABSTRACT:

Background

The number of circulating tumor cells (CTCs) in metastatic prostate cancer patients provides prognostic and predictive information. However, it is the molecular characterization of CTCs that offers insight into the biology of these tumor cells in the context of personalized treatment.

Methods

We developed a novel approach to isolate CTCs away from hematopoietic cells with high purity, enabling genomic analysis of these cells. The isolation protocol involves immunomagnetic enrichment followed by fluorescence activated cell sorting (IE/FACS). To evaluate the feasibility of isolation of CTCs by IE/FACS and downstream genomic profiling, we conducted a pilot study in patients with metastatic castration resistant prostate cancer (CRPC). Twenty (20) sequential CRPC patie

SUBMITTER: Magbanua MJ 

PROVIDER: S-EPMC3395839 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature