Respiratory syncytial virus assembles into structured filamentous virion particles independently of host cytoskeleton and related proteins.

ABSTRACT: Respiratory syncytial virus (RSV) is a single-stranded RNA virus that assembles into viral filaments at the cell surface. Virus assembly often depends on the ability of a virus to use host proteins to accomplish viral tasks. Since the fusion protein cytoplasmic tail (FCT) is critical for viral filamentous assembly, we hypothesized that host proteins important for viral assembly may be recruited by the FCT. Using a yeast two-hybrid screen, we found that filamin A interacted with FCT, and mammalian cell experiments showed it localized to viral filaments but did not affect viral replication. Furthermore, we found that a number of actin-associated proteins also were excluded from viral filaments. Actin or tubulin cytoskeletal rearrangement was not necessary for F trafficking to the cell surfac

SUBMITTER: Shaikh FY 

PROVIDER: S-EPMC3396619 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature