Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation.

ABSTRACT: Macrophages are central players in immune response, manifesting divergent phenotypes to control inflammation and innate immunity through release of cytokines and other signaling factors. Recently, the focus on metabolism has been reemphasized as critical signaling and regulatory pathways of human pathophysiology, ranging from cancer to aging, often converge on metabolic responses. Here, we used genome-scale modeling and multi-omics (transcriptomics, proteomics, and metabolomics) analysis to assess metabolic features that are critical for macrophage activation. We constructed a genome-scale metabolic network for the RAW 264.7 cell line to determine metabolic modulators of activation. Metabolites well-known to be associated with immunoactivation (glucose and arginine) and immunosuppression (

SUBMITTER: Bordbar A 

PROVIDER: S-EPMC3397418 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature