Separation of cis-trans phospholipid isomers using reversed phase LC with high resolution MS detection.

ABSTRACT: The increased presence of synthetic trans fatty acids into western diets has been shown to have deleterious effects on physiology and raising an individual's risk of developing metabolic disease, cardiovascular disease, and stroke. The importance of these fatty acids for health and the diversity of their (patho) physiological effects suggest that not only should the free trans fatty acids be studied but also monitoring the presence of these fats into the side chains of biological lipids, such as glycerophospholipids, is also essential. We developed a high resolution LC-MS method that quantitatively monitors the major lipid classes found in biospecimens in an efficient, sensitive, and robust manner while also characterizing individual lipid side chains through the use of high energy collisi

SUBMITTER: Bird SS 

PROVIDER: S-EPMC3397781 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature