Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA.

ABSTRACT: Ribosomal RNA gene transcription, co-transcriptional processing, and ribosome biogenesis are highly coordinated processes that are tightly regulated during cell growth. In this study we discovered that Mybbp1a is associated with both the RNA polymerase I complex and the ribosome biogenesis machinery. Using a reporter assay that uncouples transcription and RNA processing, we show that Mybbp1a represses rRNA gene transcription. In addition, overexpression of the protein reduces RNA polymerase I loading on endogenous rRNA genes as revealed by chromatin immunoprecipitation experiments. Accordingly, depletion of Mybbp1a results in an accumulation of the rRNA precursor in vivo but surprisingly also causes growth arrest of the cells. This effect can be explained by the observation that the modula

SUBMITTER: Hochstatter J 

PROVIDER: S-EPMC3397863 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature