Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis.

ABSTRACT:

Background

Many trypanosomatid protozoa are important human or animal pathogens. The well defined morphology and precisely choreographed division of trypanosomatid cells makes morphological analysis a powerful tool for analyzing the effect of mutations, chemical insults and changes between lifecycle stages. High-throughput image analysis of micrographs has the potential to accelerate collection of quantitative morphological data. Trypanosomatid cells have two large DNA-containing organelles, the kinetoplast (mitochondrial DNA) and nucleus, which provide useful markers for morphometric analysis; however they need to be accurately identified and often lie in close proximity. This presents a technical challenge. Accurate identification and quantitation of the DNA content of these orga

SUBMITTER: Wheeler RJ 

PROVIDER: S-EPMC3398262 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature