Characterization of resistance to pine wood nematode infection in Pinus thunbergii using suppression subtractive hybridization.

ABSTRACT:

Background

Pine wilt disease is caused by the pine wood nematode, Bursaphelenchus xylophilus, which threatens pine forests and forest ecosystems worldwide and causes serious economic losses. In the 40 years since the pathogen was identified, the physiological changes occurring as the disease progresses have been characterized using anatomical and biochemical methods, and resistant trees have been selected via breeding programs. However, no studies have assessed the molecular genetics, e.g. transcriptional changes, associated with infection-induced physiological changes in resistant or susceptible trees.

Results

We constructed seven subtractive suppression hybridization (SSH) cDNA libraries using time-course sampling of trees inoculated with pine wood nematode at 1, 3, or 7 d

SUBMITTER: Hirao T 

PROVIDER: S-EPMC3398268 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature