Repression of genes involved in melanocyte differentiation in uveal melanoma.

ABSTRACT:

Purpose

Uveal melanoma (UM) has been the subject of intense interest due to its distinctive metastatic pattern, which involves hematogenous dissemination of cancerous cells toward the liver in 50% of patients. To search for new UM prognostic markers, the Suppressive Subtractive Hybridization (SSH) technique was used to isolate genes that are differentially expressed between UM primary tumors and normal uveal melanocytes (UVM).

Methods

A subtracted cDNA library was prepared using cDNA from uncultured UM primary tumors and UVM. The expression level of selected genes was further validated by cDNA microarray, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and immunofluorescence analyses.

Results

One hundred-fifteen genes were identified using

SUBMITTER: Bergeron MA 

PROVIDER: S-EPMC3398504 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature