Immobilization of β-galactosidase onto functionalized graphene nano-sheets using response surface methodology and its analytical applications.

ABSTRACT:

Background

β-Galactosidase is a vital enzyme with diverse application in molecular biology and industries. It was covalently attached onto functionalized graphene nano-sheets for various analytical applications based on lactose reduction.

Methodology/principal findings

Response surface methodology based on Box-Behnken design of experiment was used for determination of optimal immobilization conditions, which resulted in 84.2% immobilization efficiency. Native and immobilized functionalized graphene was characterized with the help of transmission and scanning electron microscopy, followed by Fourier transform infrared (FTIR) spectroscopy. Functionalized graphene sheets decorated with islands of immobilized enzyme were evidently visualized under both transmission and scanning

SUBMITTER: Kishore D 

PROVIDER: S-EPMC3399898 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature