The oxidative DNA lesions 8,5'-cyclopurines accumulate with aging in a tissue-specific manner.

ABSTRACT: Accumulation of DNA damage is implicated in aging. This is supported by the fact that inherited defects in DNA repair can cause accelerated aging of tissues. However, clear-cut evidence for DNA damage accumulation in old age is lacking. Numerous studies report measurement of DNA damage in nuclear and mitochondrial DNA from tissues of young and old organisms, with variable outcomes. Variability results from genetic differences between specimens or the instability of some DNA lesions. To control these variables and test the hypothesis that elderly organisms have more oxidative DNA damage than young organisms, we measured 8,5'-cyclopurine-2'-deoxynucleosides (cPu), which are relatively stable, in tissues of young and old wild-type and congenic progeroid mice. We found that cPu accumulate spon

SUBMITTER: Wang J 

PROVIDER: S-EPMC3399950 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature