A method for prediction of the locations of linker regions within large multifunctional proteins, and application to a type I polyketide synthase.

ABSTRACT: Multifunctional proteins often appear to result from fusion of smaller proteins and in such cases typically can be separated into their ancestral components simply by cleaving the linker regions that separate the domains. Though possibly guided by sequence alignment, structural evidence, or light proteolysis, determination of the locations of linker regions remains empirical. We have developed an algorithm, named UMA, to predict the locations of linker regions in multifunctional proteins by quantification of the conservation of several properties within protein families, and the results agree well with structurally characterized proteins. This technique has been applied to a family of fungal type I iterative polyketide synthases (PKS), allowing prediction of the locations of all of the sta

SUBMITTER: Udwary DW 

PROVIDER: S-EPMC3400148 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature