Chemical composition and sulfur speciation in bulk tissue by x-ray spectroscopy and x-ray microscopy: corneal development during embryogenesis.

ABSTRACT: The chemical composition and sulfur (S) speciation of developing chick corneas at embryonic days 12, 14, and 16 were investigated using synchrotron scanning x-ray fluorescence microscopy and x-ray absorption near-edge structure spectroscopy. The aim was to develop techniques for the analysis of bulk tissue and identify critical physicochemical variations that correlate with changes in corneal structure-function relationships. Derived data were subjected to principal component analysis and linear discriminant analysis, which highlighted differences in the elemental and S species composition at different stages of embryonic growth. Notably, distinct elemental compositions of chlorine, potassium, calcium, phosphorus, and S altered with development during the transition of the immature opaque

SUBMITTER: Koudouna E 

PROVIDER: S-EPMC3400774 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature