Identification of candidate genes with pro-apoptotic properties by functional screening of randomly fragmented cDNA libraries.

ABSTRACT: The sequences of many genomes are available; therefore, relevant methods are needed for rapid and efficient identification of functional genes. The ability of tumour cells to resist apoptosis induced by anticancer agents may decide about the success or failure of tumour elimination. Although the CD95-signaling pathway is functional in tumour cells, the increased resistance of tumour cells to CD95-mediated apoptosis has been widely reported. In order to identify genes that might determine the response of tumour cells to CD95-mediated apoptosis, we modified the conventional technical knock out (TKO) strategy for isolation of genes that function in CD95-mediated apoptosis. Due to the fact that multiple different plasmids are usually introduced into the same cells, the effectiveness of the con

SUBMITTER: Hassan M 

PROVIDER: S-EPMC3401000 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature