MRMaid: The SRM Assay Design Tool for Arabidopsis and Other Species.

ABSTRACT: Selected reaction monitoring (SRM), sometimes called multiple reaction monitoring (MRM), is becoming the tool of choice for targeted quantitative proteomics in the plant science community. Key to a successful SRM experiment is prior identification of the distinct peptides for the proteins of interest and the determination of the so-called transitions that can be programmed into an LC-MS/MS to monitor those peptides. The transition for a given peptide comprises the intact peptide m/z and a high intensity product ion that can be monitored at a characteristic retention time (RT). To aid the design of SRM experiments, several online tools and databases have been produced to help researchers select transitions for their proteins of interest, but many of these tools are limited to the most popul

SUBMITTER: Fan J 

PROVIDER: S-EPMC3401051 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature