Systematic metabolic engineering for improvement of glycosylation efficiency in Escherichia coli.

ABSTRACT: Recently, efforts to increase the toolkit which Escherichia coli cells possess for recombinant protein production in industrial applications, has led to steady progress towards making glycosylated therapeutic proteins. Although the desire to make therapeutically relevant complex proteins with elaborate human-type glycans is a major goal, the relatively poor efficiency of the N-glycosylation process of foreign proteins in E. coli remains a hindrance for industry take-up. In this study, a systematic approach was used to increase glycoprotein production titres of an exemplar protein, AcrA, and the resulting glycosylation efficiency was quantified using a combination of Western blots and pseudo Selective Reaction Monitoring (pSRM). Western blot and pSRM results demonstrate that codon optimisin

SUBMITTER: Pandhal J 

PROVIDER: S-EPMC3401370 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature