Fast production of homogeneous recombinant RNA--towards large-scale production of RNA.

ABSTRACT: In the past decades, RNA molecules have emerged as important players in numerous cellular processes. To understand these processes at the molecular and atomic level, large amounts of homogeneous RNA are required for structural, biochemical and pharmacological investigations. Such RNAs are generally obtained from laborious and costly in vitro transcriptions or chemical synthesis. In 2007, a recombinant RNA technology has been described for the constitutive production of large amounts of recombinant RNA in Escherichia coli using a tRNA-scaffold approach. We demonstrate a general applicable extension to the described approach by introducing the following improvements: (i) enhanced transcription of large recombinant RNAs by T7 RNA polymerase (high transcription rates, versatile), (ii) efficien

SUBMITTER: Nelissen FH 

PROVIDER: S-EPMC3401473 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature