Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range.

ABSTRACT: Protein chips are widely used for high-throughput proteomic analysis, but to date, the low sensitivity and narrow dynamic range have limited their capabilities in diagnostics and proteomics. Here we present protein microarrays on a novel nanostructured, plasmonic gold film with near-infrared fluorescence enhancement of up to 100-fold, extending the dynamic range of protein detection by three orders of magnitude towards the fM regime. We employ plasmonic protein microarrays for the early detection of a cancer biomarker, carcinoembryonic antigen, in the sera of mice bearing a xenograft tumour model. Further, we demonstrate a multiplexed autoantigen array for human autoantibodies implicated in a range of autoimmune diseases with superior signal-to-noise ratios and broader dynamic range compar

SUBMITTER: Tabakman SM 

PROVIDER: S-EPMC3402035 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature