ProTeOn and proTeOff, new protein devices that inducibly activate bacterial gene expression.

ABSTRACT: Using an original workflow, we have modeled, constructed, and characterized two new molecular devices that inducibly activate gene expression in Escherichia coli. The devices, prokaryotic-TetOn and prokaryotic-TetOff, were built by fusing an inducible DNA-binding protein domain to a transcription activation domain and constructing a complementary synthetic promoter sequence through which they could control downstream gene expression. In particular, the transactivators were built using variants of the tetracycline repressor, TetR, and the transactivating domain of the LuxR activator. The complementary promoter sequence included TetR's operator, tetO, and elements of the lux promoter. These specific protein domains and their operator sites were chosen as they have been thoroughly studied and

SUBMITTER: Volzing K 

PROVIDER: S-EPMC3402092 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature