A human thymic epithelial cell culture system for the promotion of lymphopoiesis from hematopoietic stem cells.

ABSTRACT:

Objective

A human thymic epithelial cell (TEC) line expressing human leukocyte antigen-ABC and human leukocyte antigen-DR was engineered to overexpress murine Delta-like 1 (TEC-Dl1) for the purpose of establishing a human culture system that supports T lymphopoiesis from hematopoietic progenitor cells (HPCs).

Materials and methods

Cord blood or bone marrow HPCs were co-cultured with either the parental TEC line expressing low levels of the Notch ligands, Delta-like 1 and Delta-like 4, or with TEC-Dl1 to determine if these cell lines support human lymphopoiesis.

Results

In co-cultures with cord blood or bone marrow HPCs, TEC-Dl1 cells promote de novo generation of CD7(pos)CD1a(pos) T-lineage committed cells. Most CD7(pos)CD1a(hi) cells are CD4(pos)CD8(pos) double-posit

SUBMITTER: Beaudette-Zlatanova BC 

PROVIDER: S-EPMC3402177 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature