Unknown

Dataset Information

0

Position-dependent FUS-RNA interactions regulate alternative splicing events and transcriptions.


ABSTRACT: FUS is an RNA-binding protein that regulates transcription, alternative splicing, and mRNA transport. Aberrations of FUS are causally associated with familial and sporadic ALS/FTLD. We analyzed FUS-mediated transcriptions and alternative splicing events in mouse primary cortical neurons using exon arrays. We also characterized FUS-binding RNA sites in the mouse cerebrum with HITS-CLIP. We found that FUS-binding sites tend to form stable secondary structures. Analysis of position-dependence of FUS-binding sites disclosed scattered binding of FUS to and around the alternatively spliced exons including those associated with neurodegeneration such as Mapt, Camk2a, and Fmr1. We also found that FUS is often bound to the antisense RNA strand at the promoter regions. Global analysis of these FUS-tags and the expression profiles disclosed that binding of FUS to the promoter antisense strand downregulates transcriptions of the coding strand. Our analysis revealed that FUS regulates alternative splicing events and transcriptions in a position-dependent manner.

SUBMITTER: Ishigaki S 

PROVIDER: S-EPMC3402842 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

altmetric image

Publications

Position-dependent FUS-RNA interactions regulate alternative splicing events and transcriptions.

Ishigaki Shinsuke S   Masuda Akio A   Fujioka Yusuke Y   Iguchi Yohei Y   Katsuno Masahisa M   Shibata Akihide A   Urano Fumihiko F   Sobue Gen G   Ohno Kinji K  

Scientific reports 20120724


FUS is an RNA-binding protein that regulates transcription, alternative splicing, and mRNA transport. Aberrations of FUS are causally associated with familial and sporadic ALS/FTLD. We analyzed FUS-mediated transcriptions and alternative splicing events in mouse primary cortical neurons using exon arrays. We also characterized FUS-binding RNA sites in the mouse cerebrum with HITS-CLIP. We found that FUS-binding sites tend to form stable secondary structures. Analysis of position-dependence of FU  ...[more]

Similar Datasets

| S-EPMC2933513 | biostudies-literature
| S-EPMC3973337 | biostudies-literature
2010-09-01 | E-GEOD-23522 | biostudies-arrayexpress
2014-01-22 | E-GEOD-53354 | biostudies-arrayexpress
2010-09-01 | E-GEOD-23513 | biostudies-arrayexpress
2010-09-01 | GSE23522 | GEO
2014-01-22 | GSE53354 | GEO
2010-09-01 | E-GEOD-23514 | biostudies-arrayexpress
2010-09-01 | GSE23513 | GEO
2010-09-01 | GSE23514 | GEO