Enzymatic oxygen scavenging for photostability without pH drop in single-molecule experiments.

ABSTRACT: Over the past years, bottom-up bionanotechnology has been developed as a promising tool for future technological applications. Many of these biomolecule-based assemblies are characterized using various single-molecule techniques that require strict anaerobic conditions. The most common oxygen scavengers for single-molecule experiments are glucose oxidase and catalase (GOC) or protocatechuate dioxygenase (PCD). One of the pitfalls of these systems, however, is the production of carboxylic acids. These acids can result in a significant pH drop over the course of experiments and must thus be compensated by an increased buffer strength. Here, we present pyranose oxidase and catalase (POC) as a novel enzymatic system to perform single-molecule experiments in pH-stable conditions at arbitrary bu

SUBMITTER: Swoboda M 

PROVIDER: S-EPMC3403312 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature