Designing specific protein-protein interactions using computation, experimental library screening, or integrated methods.

ABSTRACT: Given the importance of protein-protein interactions for nearly all biological processes, the design of protein affinity reagents for use in research, diagnosis or therapy is an important endeavor. Engineered proteins would ideally have high specificities for their intended targets, but achieving interaction specificity by design can be challenging. There are two major approaches to protein design or redesign. Most commonly, proteins and peptides are engineered using experimental library screening and/or in vitro evolution. An alternative approach involves using protein structure and computational modeling to rationally choose sequences predicted to have desirable properties. Computational design has successfully produced novel proteins with enhanced stability, desired interactions and enz

SUBMITTER: Chen TS 

PROVIDER: S-EPMC3403433 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature