Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis.

ABSTRACT: BacD is an ATP-dependent dipeptide ligase responsible for the biosynthesis of L-alanyl-L-anticapsin, a precursor of an antibiotic produced by Bacillus spp. In contrast to the well-studied and phylogenetically related D-alanine: D-alanine ligase (Ddl), BacD synthesizes dipeptides using L-amino acids as substrates and has a low substrate specificity in vitro. The enzyme is of great interest because of its potential application in industrial protein engineering for the environmentally friendly biological production of useful peptide compounds, such as physiologically active peptides, artificial sweeteners and antibiotics, but the determinants of its substrate specificity and its catalytic mechanism have not yet been established due to a lack of structural information. In this study, we report

SUBMITTER: Shomura Y 

PROVIDER: S-EPMC3403468 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature