Multiphoton optical image guided spectroscopy method for characterization of collagen-based materials modified by glycation.

ABSTRACT: The cross-linking with reducing sugars, known as glycation, is used to increase stiffness and strength of tissues and artificial collagen-based scaffolds. Nondestructive characterization methods that report on the structures within these materials could clarify the effects of glycation. For doing this nondestructive evaluation, we employed an in situ one-photon fluorescence as well as multiphoton microscopy method that combined two-photon fluorescence and second harmonic generation signals. We incubated collagen hydrogels with glyceraldehyde, ribose, and glucose and observed an increase in the in situ fluorescence and structural alterations within the materials during the course of glycation. The two-photon fluorescence emission maximum was observed at about 460 nm. The fluorescence emissi

SUBMITTER: Hwang YJ 

PROVIDER: S-EPMC3403829 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature